Language: English Japanese

Genomic Services

Custom Genome Sequencing, Closing, and Annotation.

Igenbio has an exceptional track record in genome closing and custom sequencing. We have nearly two decades experience sequencing and closing microbial genomes of industrial and scientific significance. Igenbio, Inc. leverages the power of ERGO™'s best in class genomic sequence database and our unique knowledge of chromosomal clustering to rapidly perform intelligently targeted gap closure.
Complete and Published Genomes closed using ERGO™
  • Aster Yellows Phytoplasma
  • Bacillus cereus ATCC14579
  • Brucella melitensis 16M
  • Chlamydia trachomatis A/HAR13
  • Fusobacterium nucleatum subsp. nucleatum ATCC 25586
  • Granulibacter bethesdensis CGDNIH1
  • Streptococcus pyogenes MGAS10394
  • Streptococcus pyogenes MGAS6180
  • Streptococcus pyogenes MGAS10270
  • Streptococcus pyogenes MGAS10750
  • Streptococcus pyogenes MGAS2096
  • Streptococcus pyogenes MGAS5005
  • Streptococcus pyogenes MGAS9429
  • and many more...

Metabolic Engineering and Reconstruction

Industries, such as energy and fermentation companies, benefit from leveraging ERGO™'s powerful ability to deduce the metabolic reconstruction of the core functionality of a whole organism from genome sequence data. Traditional wet-lab experiments would take decades to reproduce what is available immediately in ERGO™, enabling Scientists and researchers to accelerate their research. Igenbio, Inc. guides and improves strain engineering by performing a comprehensive analysis of the organism's genome and metabolome, and devising a blueprint for strain improvement and optimization.
  • Improve throughput and product yield
  • Develop production pathways
  • Avoid competitors intellectual property

Genome Analysis Services

Automated Annotation and Analysis

You are done sequencing - now, where are the genes and what do they do? Igenbio, Inc. automatically annotates and analyzes genomes, identifying the genes and RNAs, assigning functions to the identified proteins and RNAs, and link the functions to the network of pathways in the organism. Typically ERGO™ automated annotation results in 10% to 20% more successful function prediction than competing technologies.

Manual annotation

Reviewing and curating the automated annotation results improves the accuracy, adds more functional predictions for unassigned genes and makes additional connections to ERGO™'s pathway database. Manual annotation by Igenbio's scientists is an excellent way to refine a metabolic model for an organism by identifying missing steps in critical biochemical pathways and generating the most accurate gene functions.

Physiological analysis

Igenbio's scientists using ERGO™ create complete metabolic reconstructions of microorganisms and work with customers analyzing the metabolic and non-metabolic pathways to discern organism physiology. Igenbio's scientists have designed growth media for fastidious growing intracellular plant and animal pathogens.

Genome comparison

Using ERGO™, our scientists work with you to explore the genomic basis of unique physiology and shared physiology between microorganisms or between strains. High-resolution genome comparison identifies SNPs that affect species and strain differences. This is a powerful tool for improving production of bacterial strains and aiding bioprocess development.

Data-mining for target gene discovery

Finding unique genes for drug discovery or metabolic engineering targets is a critical start in any research project, since in silico analysis fast-tracks research and saves weeks or months of laboratory time. Data mining can also identify the genes that encode novel or useful functions in bioproduction patents.

Microarray Design

One benefit of the decoding of genome sequence is the ability to start performing gene expression experimentation that can help elucidate the function genes and the pathways they participate in. Igenbio, Inc. can accelerate the time from sequencing a genome to performing expression-based research through the design of microarrays. Igenbio, Inc. can design single genome or composite genome arrays to enable the understanding of the organism or the behavior of the organism with its host. Once the microarray data has been generated, the rich annotation information provided in ERGO™ is invaluable in the interpretation of the experimental results.
Related Genomes & Publications
  • Yersinia enterocolitica: http://www.ncbi.nlm.nih.gov/pubmed/15256571
  • Pichia pastoris: http://www.ncbi.nlm.nih.gov/pubmed?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17578563

Growth Media Design

Some of the most exciting and commercially interesting microorganisms including attenuated vaccine strains are often difficult to grow at the scale or efficiency needed for their research or operational uses. While the field of microbiology has generated a large volume of genome sequence data over the past 16 years, the genome on its own is insufficient to understand what media is needed to culture these difficult-to-grow organisms. Igenbio, Inc. can design growth media for many of these difficult-to-grow organisms through metabolic analysis of the organism. This capability has evolved out of many years of work in the field of microbiology, and a number of customer successes.
Related Genomes & Publications
  • Staphylococcus aureus: http://www.ncbi.nlm.nih.gov/pubmed/19376871
  • Xylella fastidiosa: http://www.ncbi.nlm.nih.gov/pubmed/12368248, http://www.ncbi.nlm.nih.gov/pubmed/12205291

Learn more about how Igenbio's technology can help your organization...